The detection of alkylation damage in the DNA of human gastrointestinal tissues.
Affiliation
Department of Surgery, Wythenshawe Hospital, Manchester.Issue Date
1991-07
Metadata
Show full item recordAbstract
Damage arising from putative environmental sources has been found in the DNA of the gastric and colorectal mucosae of patients presenting with gastrointestinal disorders from the South Manchester area. O6-Methylguanine (O6-MeG) in the range 0.010- greater than 0.300 mu moles mole-1 adenine was heterogeneously distributed both between and within individuals. The pattern of alkylation of tissue DNA appears to differ when comparison is made between gastric and colorectal samples. Most of the gastric tumour DNA samples were alkylated (5/6; 0.087 +/- 0.097), whereas the DNA of the associated mucosa was alkylated less frequently (2/7) and to a lesser extent; (0.017 +/- 0.030; P = 0.07). Conversely, colorectal tumour DNA was alkylated infrequently (1/7) and to a lower extent (0.003 +/- 0.007) than the DNA of the adjacent mucosa (8/10 samples alkylated with a mean of 0.083 +/- 0.106; P = less than 0.01), or indeed of any other tissue. Although increased levels of DNA damage in tissue associated with malignant disease have been indicated by independent studies of DNA damage at other cancer sites, significant differences were not observed in the present report, neither was there any suggestion of a relationship with smoking or alcohol consumption. The data provided by this report indicate that exposure to putative environmental alkylating agents occurs in the UK at levels comparable to those previously detected in areas of higher cancer risk. Although we cannot determine the extent to which this DNA damage is attributable to normal background exposures, it is evident that the alkylation of tissue DNA occurs and is not uniform. In conjunction with other reports, therefore these differences may begin to provide indications of mechanisms that could be of relevance in the aetiology of gastrointestinal cancers.Citation
The detection of alkylation damage in the DNA of human gastrointestinal tissues. 1991, 64 (1):59-63 Br. J. CancerJournal
British Journal of CancerPubMed ID
1854628Type
ArticleLanguage
enISSN
0007-0920Collections
Related articles
- The enteroendocrine cells during the normal development of the rat and in some human digestive diseases.
- Authors: Taşcă C, Secăreanu-Chelaru E, Budu S
- Issue date: 1978 Jul-Sep
- DNA alkylation and repair in the large bowel: animal and human studies.
- Authors: Povey AC, Badawi AF, Cooper DP, Hall CN, Harrison KL, Jackson PE, Lees NP, O'Connor PJ, Margison GP
- Issue date: 2002 Nov
- Tissue and cell specific methylation, repair and synthesis of DNA in the upper gastrointestinal tract of Wistar rats treated with single doses of N-methyl-N'-nitro-N-nitrosoguanidine.
- Authors: Zaidi NH, Potten CS, Margison GP, Cooper DP, O'Connor PJ
- Issue date: 1993 Oct
- Studies in gastric carcinogenesis. IV. O6-methylguanine and its repair in normal and atrophic biopsy specimens of human gastric mucosa. Correlation of O6-alkylguanine-DNA alkyltransferase activities in gastric mucosa and circulating lymphocytes.
- Authors: Kyrtopoulos SA, Ampatzi P, Davaris P, Haritopoulos N, Golematis B
- Issue date: 1990 Mar
- Mucins and mucosal protection in the gastrointestinal tract: new prospects for mucins in the pathology of gastrointestinal disease.
- Authors: Corfield AP, Myerscough N, Longman R, Sylvester P, Arul S, Pignatelli M
- Issue date: 2000 Oct