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dc.contributor.authorJust, U
dc.contributor.authorStocking, C
dc.contributor.authorSpooncer, Elaine
dc.contributor.authorDexter, T Michael
dc.contributor.authorOstertag, W
dc.date.accessioned2010-06-11T08:58:19Z
dc.date.available2010-06-11T08:58:19Z
dc.date.issued1991-03-22
dc.identifier.citationExpression of the GM-CSF gene after retroviral transfer in hematopoietic stem cell lines induces synchronous granulocyte-macrophage differentiation. 1991, 64 (6):1163-73 Cellen
dc.identifier.issn0092-8674
dc.identifier.pmid2004422
dc.identifier.doi10.1016/0092-8674(91)90271-Y
dc.identifier.urihttp://hdl.handle.net/10541/104658
dc.description.abstractMultipotent murine stem cell lines (FDC-Pmix) depend on IL-3 for self-renewal and proliferation and can be induced to differentiate into multiple hematopoietic lineages. Single FDC-Pmix cells infected with retroviral vectors expressing GM-CSF are induced to differentiate into granulocytes and macrophages. This results in a complete loss of clonogenic cells if IL-3 is not exogenously supplied; however, multipotent variants can be selected that do not terminally differentiate if cells are kept in the presence of IL-3. Unidirectional and synchronous granulocyte and macrophage differentiation accompanied with loss of self-renewal capacity is induced when IL-3 is removed. Our data indicate that activation of the GM-CSF receptor induces differentiation of stem cells by an instructive mechanism that can be blocked by the activated IL-3 receptor. A model of how receptors can induce proliferation and cell-specific differentiation by two separate pathways is discussed.
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals
dc.subject.meshCell Differentiation
dc.subject.meshCell Division
dc.subject.meshCell Line
dc.subject.meshGenetic Vectors
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor
dc.subject.meshGranulocytes
dc.subject.meshHematopoietic Stem Cells
dc.subject.meshInterleukin-3
dc.subject.meshMacrophages
dc.subject.meshMice
dc.subject.meshMutation
dc.subject.meshRetroviridae
dc.titleExpression of the GM-CSF gene after retroviral transfer in hematopoietic stem cell lines induces synchronous granulocyte-macrophage differentiation.en
dc.typeArticleen
dc.contributor.departmentHeinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Federal Republic of Germany.en
dc.identifier.journalCellen
html.description.abstractMultipotent murine stem cell lines (FDC-Pmix) depend on IL-3 for self-renewal and proliferation and can be induced to differentiate into multiple hematopoietic lineages. Single FDC-Pmix cells infected with retroviral vectors expressing GM-CSF are induced to differentiate into granulocytes and macrophages. This results in a complete loss of clonogenic cells if IL-3 is not exogenously supplied; however, multipotent variants can be selected that do not terminally differentiate if cells are kept in the presence of IL-3. Unidirectional and synchronous granulocyte and macrophage differentiation accompanied with loss of self-renewal capacity is induced when IL-3 is removed. Our data indicate that activation of the GM-CSF receptor induces differentiation of stem cells by an instructive mechanism that can be blocked by the activated IL-3 receptor. A model of how receptors can induce proliferation and cell-specific differentiation by two separate pathways is discussed.


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