The decay of autoradiographic grain number over crypt base columnar cells in murine ileum as a measure of their generation time.
Affiliation
Department of Pathophysiology, Institute of Rheumatology, Warsaw, Poland.Issue Date
1991-01
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The decay in the number of grains over [3H]-thymidine labelled crypt base columnar cells (BCC) in autoradiographs of the ileum of BDF1 mice has been studied. The results revealed that using the conventional grain count halving (GCH) method it is possible to obtain an estimation of the generation time (Tc) of the proliferative BCC cells in the Paneth cell zone (PC-zone) of 18.8 +/- 0.74 h. This lies within the range obtained by the percent labelled mitoses (PLM) method, but is shorter than most values obtained by stathmokinetic methods. The present data show no evidence for a shortening of the cell cycle 3 days after irradiation (8 Gy) which is contrary to some earlier observations. Some reasons for this discrepancy are discussed. The comparatively high labelling index of the BCC allows a larger amount of data to be easily collected, compared with the PLM technique, and correction factors which take into account the complicated shape of the bottom of the crypt are not required.Citation
The decay of autoradiographic grain number over crypt base columnar cells in murine ileum as a measure of their generation time. 1991, 24 (1):21-31 Cell Prolif.Journal
Cell ProliferationDOI
10.1111/j.1365-2184.1991.tb01508.xPubMed ID
2009314Type
ArticleLanguage
enISSN
0960-7722ae974a485f413a2113503eed53cd6c53
10.1111/j.1365-2184.1991.tb01508.x
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