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dc.contributor.authorLees, Janice F
dc.contributor.authorArrand, Jane E
dc.contributor.authorPepper, Stuart D
dc.contributor.authorStewart, James P
dc.contributor.authorMackett, Mike
dc.contributor.authorArrand, John R
dc.date.accessioned2010-06-09T16:04:16Z
dc.date.available2010-06-09T16:04:16Z
dc.date.issued1993-08
dc.identifier.citationThe Epstein-Barr virus candidate vaccine antigen gp340/220 is highly conserved between virus types A and B. 1993, 195 (2):578-86 Virologyen
dc.identifier.issn0042-6822
dc.identifier.pmid8393237
dc.identifier.urihttp://hdl.handle.net/10541/104512
dc.description.abstractAnti-Epstein-Barr Virus (EBV) vaccines are being developed which are based on the gp340/220 membrane antigen (MA) gene products from the B95-8 strain. Some proteins are known to be immunologically quite different between type-A (1) and type-B (2) strains of EBV and therefore from a vaccine point of view it was critical to evaluate the degree of conservation of gp340/220. The complete MA coding sequence was determined for two B-type viruses, AG876 and P3HR-1, for comparison with the A-type B95-8. A variable region within MA was sequenced from several other strains. In addition the other open reading frames within the MA-containing BamHI-L fragment of AG876 were sequenced and compared. The results show that there is a high degree of homology between all strains examined. Although some differences were found within the MA coding sequence the only major site of variation was within the repeat region and no consistent A/B changes were found. Monoclonal antibodies generated against A-type MA and representing six epitope groups along the length of the gp340 molecule were found to recognize B-type gp340, thereby demonstrating functional homology. We conclude that, as a vaccine antigen, B95-8 gp340/220 should be equally effective against both types of EBV.
dc.language.isoenen
dc.subject.meshAmino Acid Sequence
dc.subject.meshAntibodies, Monoclonal
dc.subject.meshAntigens, Viral
dc.subject.meshBase Sequence
dc.subject.meshCell Line
dc.subject.meshConserved Sequence
dc.subject.meshDNA, Viral
dc.subject.meshFluorescent Antibody Technique
dc.subject.meshHerpesvirus 4, Human
dc.subject.meshHumans
dc.subject.meshMolecular Sequence Data
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshSequence Homology, Amino Acid
dc.subject.meshViral Matrix Proteins
dc.subject.meshViral Vaccines
dc.titleThe Epstein-Barr virus candidate vaccine antigen gp340/220 is highly conserved between virus types A and B.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Laboratories, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester M20 9BX, United Kingdom.en
dc.identifier.journalVirologyen
html.description.abstractAnti-Epstein-Barr Virus (EBV) vaccines are being developed which are based on the gp340/220 membrane antigen (MA) gene products from the B95-8 strain. Some proteins are known to be immunologically quite different between type-A (1) and type-B (2) strains of EBV and therefore from a vaccine point of view it was critical to evaluate the degree of conservation of gp340/220. The complete MA coding sequence was determined for two B-type viruses, AG876 and P3HR-1, for comparison with the A-type B95-8. A variable region within MA was sequenced from several other strains. In addition the other open reading frames within the MA-containing BamHI-L fragment of AG876 were sequenced and compared. The results show that there is a high degree of homology between all strains examined. Although some differences were found within the MA coding sequence the only major site of variation was within the repeat region and no consistent A/B changes were found. Monoclonal antibodies generated against A-type MA and representing six epitope groups along the length of the gp340 molecule were found to recognize B-type gp340, thereby demonstrating functional homology. We conclude that, as a vaccine antigen, B95-8 gp340/220 should be equally effective against both types of EBV.


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