Affiliation
Cancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.Issue Date
1993-05
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Show full item recordAbstract
A method is described for producing outgrowths of small nephron segments (average 24 cells) in culture. The method was used to estimate an overall colony-forming efficiency of 4.6% for cells constituting the segments. Efficiency was found to be lower for thick segments (1%) than for thin segments (6%) from Henle's loop. The latter higher level indicates that precursor cells are concentrated near the middle of the nephron. For comparison, a two-dose irradiation technique was used to calculate a mean number of 5 +/- 2 (SE) clonogens per segment producing outgrowths. This tended to be higher than the value of about 1 calculated from the 65% of segments producing outgrowths, as expected if the remaining segments contained no clonogens.Citation
The distribution of colony-forming cells in the kidney. 1993, 26 (3):263-9 Cell Prolif.Journal
Cell ProliferationDOI
10.1111/j.1365-2184.1993.tb00024.xPubMed ID
8324073Type
ArticleLanguage
enISSN
0960-7722ae974a485f413a2113503eed53cd6c53
10.1111/j.1365-2184.1993.tb00024.x
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