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    The distribution of colony-forming cells in the kidney.

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    Authors
    Jen, Yee-Min
    Hendry, Jolyon H
    Affiliation
    Cancer Research Campaign Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
    Issue Date
    1993-05
    
    Metadata
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    Abstract
    A method is described for producing outgrowths of small nephron segments (average 24 cells) in culture. The method was used to estimate an overall colony-forming efficiency of 4.6% for cells constituting the segments. Efficiency was found to be lower for thick segments (1%) than for thin segments (6%) from Henle's loop. The latter higher level indicates that precursor cells are concentrated near the middle of the nephron. For comparison, a two-dose irradiation technique was used to calculate a mean number of 5 +/- 2 (SE) clonogens per segment producing outgrowths. This tended to be higher than the value of about 1 calculated from the 65% of segments producing outgrowths, as expected if the remaining segments contained no clonogens.
    Citation
    The distribution of colony-forming cells in the kidney. 1993, 26 (3):263-9 Cell Prolif.
    Journal
    Cell Proliferation
    URI
    http://hdl.handle.net/10541/101875
    DOI
    10.1111/j.1365-2184.1993.tb00024.x
    PubMed ID
    8324073
    Type
    Article
    Language
    en
    ISSN
    0960-7722
    ae974a485f413a2113503eed53cd6c53
    10.1111/j.1365-2184.1993.tb00024.x
    Scopus Count
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    All Paterson Institute for Cancer Research

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