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    MHC class II-restricted presentation of endogenously synthesized antigen: Epstein-Barr virus transformed B cell lines can present the viral glycoprotein gp340 by two distinct pathways.

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    Authors
    Lee, S P
    Wallace, L E
    Mackett, Mike
    Arrand, John R
    Searle, P F
    Rowe, M
    Rickinson, A B
    Affiliation
    Department of Cancer Studies, University of Birmingham, UK.
    Issue Date
    1993-05
    
    Metadata
    Show full item record
    Abstract
    Epstein-Barr virus (EBV) transformed B lymphoblastoid cell lines (LCL) efficiently process exogenous antigens for MHC class II-restricted presentation via the chloroquine-sensitive endosomal pathway. Using MHC class II-restricted T cell clones specific for EBV structural proteins, however, we frequently observed significant responses to autologous LCL cells without the addition of exogenous virus. Such responses were reduced by pre-treating the LCL with acyclovir (ACV), a drug blocking productive EBV infection. This suggested T cell recognition of antigen synthesized by LCL cells spontaneously entering virus productive cycle, and led us to question by what route(s) MHC class II-restricted presentation of endogenously synthesized virion proteins was occurring. Cell sorting experiments, using the viral envelope glycoprotein gp340 as a surface marker of productively-infected cells, confirmed that stimulatory activity lay within the gp340-positive fraction. However, closer analysis revealed that most of these cells were not productively-infected but were EBV receptor-positive and had bound released virus. We infer that receptor-mediated delivery of released virus into the endosomal pathway is one route whereby an LCL can present endogenously synthesized EBV proteins on MHC class II molecules. To ask whether another, more direct, route of processing was possible, we used a recombinant vaccinia viral vector to express gp340 de novo in ACV-treated LCLs. Significantly, these cells presented the endogenously synthesized antigen to autologous gp340-specific T cell clones via a chloroquine-resistant pathway. In the same experiments, vaccinia-mediated expression of a signal peptide-deleted form of gp340 did not lead to T cell stimulation, suggesting that this second route of processing required entry of endogenously synthesized antigen into the endoplasmic reticulum.
    Citation
    MHC class II-restricted presentation of endogenously synthesized antigen: Epstein-Barr virus transformed B cell lines can present the viral glycoprotein gp340 by two distinct pathways. 1993, 5 (5):451-60 Int. Immunol.
    Journal
    International Immunology
    URI
    http://hdl.handle.net/10541/101856
    DOI
    10.1093/intimm/5.5.451
    PubMed ID
    8391306
    Type
    Article
    Language
    en
    ISSN
    0953-8178
    ae974a485f413a2113503eed53cd6c53
    10.1093/intimm/5.5.451
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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