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    Multiple signaling pathways mediate anti-Ig and IL-4-induced early response gene expression in human tonsillar B cells.

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    Authors
    Murphy, J J
    Norton, John D
    Affiliation
    Division of Life Sciences, King's College London, GB.
    Issue Date
    1993-11
    
    Metadata
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    Abstract
    We have analyzed the relationship between the signaling pathways coupled to surface immunoglobulin and interleukin (IL)-4 receptors in human B cells from the patterns of expression of a panel of phorbol ester-inducible early response genes (ERG) activated by anti-IgM and IL-4 stimulation in vitro. Anti-IgM stimulation led to the induction of all eleven ERG tested. Two of these, the proto-oncogene, c-fos and an anonymous ERG 1R20 were insensitive to protein kinase C (PKC) inhibition with the drug, staurosporine and retained inducibility after down-regulation of PKC activity by purging with phorbol ester. These observations are consistent with previous data showing anti-IgM signaling through both PKC-dependent and PKC-independent pathways. c-fos and 1R20 were also the only ERG inducible in response to IL-4 stimulation and whilst ionomycin induced only c-fos, dibutyryl cyclic adenosine monophosphate stimulation led to induction of both c-fos and 1R20. These observations lend support to a role for the adenylate cyclase pathway being important for coupling of IL-4-generated signals to B cells responses. None of the anti-IgM-responsive ERG was further induced when B cells were co-stimulated with a combination of anti-IgM and IL-4, suggesting that the signaling cascades from these two agents are integrated downstream of third messenger pathways to synergistically promote B cell proliferation.
    Citation
    Multiple signaling pathways mediate anti-Ig and IL-4-induced early response gene expression in human tonsillar B cells. 1993, 23 (11):2876-81 Eur. J. Immunol.
    Journal
    European Journal of Immunology
    URI
    http://hdl.handle.net/10541/100406
    DOI
    10.1002/eji.1830231122
    PubMed ID
    7693480
    Type
    Article
    Language
    en
    ISSN
    0014-2980
    ae974a485f413a2113503eed53cd6c53
    10.1002/eji.1830231122
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

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