The expression of matrix metalloproteinases and their inhibitors by pig synovial cells and their regulation by combinations of cytokines and growth factors.
Affiliation
Cell and Molecular Biology Department, Strangeways Research Laboratory, Cambridge, U.K.Issue Date
1993-11
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1. Pig synovial fibroblasts in culture were studied to determine if they were an easily reproducible model system for studying the actions of cytokines and growth factors on human synovial cells. The biochemical analyses were conducted by activity assays, enzymography and Northern blot. 2. Human recombinant interleukin-1 alpha, basic fibroblast growth factor and transforming growth factor-beta 1 were studied in combinations because of their known involvement in controlling tissue remodelling. 3. The response of pig fibroblasts to these agents, in terms of the expression of matrix metalloproteinases (collagenase, gelatinase and stromelysin) and their inhibitors (TIMPs), show that they behave similarly enough to human cells for use when supplies of human primary cells are unavailable.Citation
The expression of matrix metalloproteinases and their inhibitors by pig synovial cells and their regulation by combinations of cytokines and growth factors. 1993, 106 (3):691-704 Comp. Biochem. Physiol., BJournal
Comparative Biochemistry and Physiology. B, Comparative BiochemistryDOI
10.1016/0305-0491(93)90151-TPubMed ID
8281764Type
ArticleLanguage
enISSN
0305-0491ae974a485f413a2113503eed53cd6c53
10.1016/0305-0491(93)90151-T
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