A polymerase chain reaction-based method for the detection of trisomy 12 in B chronic lymphocytic leukaemia.
Affiliation
CRC Department of Gene Regulation, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, U.K.Issue Date
1993-09
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We report a polymerase chain reaction (PCR)-based method for detection of clonal trisomy 12 cell populations in B cell chronic lymphocytic leukaemia which is particularly applicable to retrospective analysis or archival material. The method utilizes three restriction enzyme site polymorphisms linked to the KRAS2 locus on chromosome 12 to monitor allele imbalance resulting from duplication of this chromosome. 21/24 unselected chronic lymphocytic leukaemia cases (88%) were informative for at least one polymorphism and of these, eight (33% of total cases) displayed allelic imbalance consistent with trisomy 12.Citation
A polymerase chain reaction-based method for the detection of trisomy 12 in B chronic lymphocytic leukaemia. 1993, 85 (1):179-81 Br. J. Haematol.Journal
British Journal of HaematologyDOI
10.1111/j.1365-2141.1993.tb08664.xPubMed ID
8251388Type
ArticleLanguage
enISSN
0007-1048ae974a485f413a2113503eed53cd6c53
10.1111/j.1365-2141.1993.tb08664.x
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