• Login
    View Item 
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    •   Home
    • The Manchester Institute Cancer Research UK
    • All Paterson Institute for Cancer Research
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of ChristieCommunitiesTitleAuthorsIssue DateSubmit DateSubjectsThis CollectionTitleAuthorsIssue DateSubmit DateSubjectsProfilesView

    My Account

    LoginRegister

    Local Links

    The Christie WebsiteChristie Library and Knowledge Service

    Statistics

    Display statistics

    Proliferation of spleen colony forming units (CFU-S8, CFU-S13) and cells with marrow repopulating ability.

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Authors
    Lord, Brian I
    Woolford, Lorna B
    Affiliation
    CRC Department of Experimental Haematology, Paterson Institute for Cancer Research, Manchester, England, United Kingdom.
    Issue Date
    1993-05
    
    Metadata
    Show full item record
    Abstract
    The practicalities of gene transfer therapy using retroviral vector systems require both that host cells be as primitive as possible and that those cells be proliferating. Here, the kinetics of hemopoietic stem cells with marrow repopulating ability (MRA) have been studied with a view to defining the timescale over which these normally quiescent cells can be triggered into cell cycle. Mice were injected with hydroxyurea (1 g/kg) four times over a period of 26 h and assayed at intervals up to eight days for 8-day and 13-day spleen colony-forming units (CFU-S) and for generation of 12-day CFU-S in the bone marrow (MRA assay). The proliferative activity of these cell populations was measured by in vitro tritiated thymidine assays. CFU-S were reduced rapidly to 11% of normal and induced into cycle. Their number and proliferative quiescence recovered by four to five days. Cells with MRA reached their nadir after four days and only then started to proliferate. For each of these progenitor cell subclasses, the proliferative activity inversely reflects their numbers and indicates regulation by negative feedback processes.
    Citation
    Proliferation of spleen colony forming units (CFU-S8, CFU-S13) and cells with marrow repopulating ability. 1993, 11 (3):212-7 Stem Cells
    Journal
    Stem Cells
    URI
    http://hdl.handle.net/10541/100084
    DOI
    10.1002/stem.5530110308
    PubMed ID
    8318908
    Type
    Article
    Language
    en
    ISSN
    1066-5099
    ae974a485f413a2113503eed53cd6c53
    10.1002/stem.5530110308
    Scopus Count
    Collections
    All Paterson Institute for Cancer Research

    entitlement

    Related articles

    • Effect of cytokine treatment (granulocyte colony-stimulating factor and stem cell factor) on hematopoiesis and the circulating pool of hematopoietic stem cells in mice.
    • Authors: Drize N, Chertkov J, Samoilina N, Zander A
    • Issue date: 1996 Jun
    • Cells with marrow and spleen repopulating ability and forming spleen colonies on day 16, 12, and 8 are sequentially ordered on the basis of increasing rhodamine 123 retention.
    • Authors: Ploemacher RE, Brons NH
    • Issue date: 1988 Sep
    • Hematopoietic stem cells in the hereditarily anemic Belgrade laboratory (b/b) rat.
    • Authors: Ivanović Z, Milenković P, Vasiljevska M, Dekić M
    • Issue date: 1995 Oct
    • Marrow repopulating cells, but not CFU-S, establish long-term in vitro hemopoiesis on a marrow-derived stromal layer.
    • Authors: van der Sluijs JP, de Jong JP, Brons NH, Ploemacher RE
    • Issue date: 1990 Sep
    • The effects of x-irradiation on hematopoietic stem cell compartments in the mouse.
    • Authors: Meijne EI, van der Winden-van Groenewegen RJ, Ploemacher RE, Vos O, David JA, Huiskamp R
    • Issue date: 1991 Aug
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.