Medical Oncology
http://hdl.handle.net/10541/65033
2024-03-15T22:35:41ZSelected topics from the advancing forefront of glycoprotein and glycolipid research.
http://hdl.handle.net/10541/91617
Selected topics from the advancing forefront of glycoprotein and glycolipid research.
Sharon, Nathan; Gallagher, John T
2009-10-01T00:00:00ZAdding more content to screening: reactivation of FOXO as a therapeutic strategy.
http://hdl.handle.net/10541/91485
Adding more content to screening: reactivation of FOXO as a therapeutic strategy.
Zanella, Fabian; Carnero, Amancio
The discovery of novel targets that can be pharmacologically exploited to lead to a better disease outcome has long been an aim of biomedical research. At present, the technology and robotisation available have pushed the search for novel molecules to a high-throughput screening (HTS) context, making it possible to screen several hundreds of compounds or genes in a single day. High-content screenings (HCS) have added a refined complexity to the screening processes, as the information drawn from an image- based assay is more complete than the monoparametric readouts obtained in classical HTS assays. Here, we review the development of HCS platforms to identify molecules influencing FOXO nuclear relocation and activation as pharmacological targets, their applicability and the future directions of the screening field.
2009-10-01T00:00:00ZQuantifying antivascular effects of monoclonal antibodies to vascular endothelial growth factor: insights from imaging.
http://hdl.handle.net/10541/87698
Quantifying antivascular effects of monoclonal antibodies to vascular endothelial growth factor: insights from imaging.
O'Connor, James P B; Carano, Richard A D; Clamp, Andrew R; Ross, Jed; Ho, Calvin C K; Jackson, Alan; Parker, Geoff J M; Rose, Chris J; Peale, Franklin V; Friesenhahn, Michel; Mitchell, Claire L; Watson, Yvonne; Roberts, Caleb; Hope, Lynn; Cheung, Susan; Reslan, Hani Bou; Go, Mary Ann T; Pacheco, Glenn J; Wu, Xiumin; Cao, Tim C; Ross, Sarajane; Buonaccorsi, Giovanni A; Davies, Karen; Hasan, Jurjees; Thornton, Paula; Del Puerto, Olivia; Ferrara, Napoleone; Van Bruggen, Nicholas; Jayson, Gordon C
2009-01-01T00:00:00ZMethods comparison for high-resolution transcriptional analysis of archival material on Affymetrix Plus 2.0 and Exon 1.0 microarrays.
http://hdl.handle.net/10541/87535
Methods comparison for high-resolution transcriptional analysis of archival material on Affymetrix Plus 2.0 and Exon 1.0 microarrays.
Linton, Kim M; Hey, Yvonne; Dibben, Sian; Miller, Crispin J; Freemont, Anthony J; Radford, John A; Pepper, Stuart D
Microarray gene expression profiling of formalin-fixed paraffin-embedded (FFPE) tissues is a new and evolving technique. This report compares transcript detection rates on Affymetrix U133 Plus 2.0 and Human Exon 1.0 ST GeneChips across several RNA extraction and target labeling protocols, using routinely collected archival FFPE samples. All RNA extraction protocols tested (Ambion-Optimum, Ambion-RecoverAll, and Qiagen-RNeasy FFPE) provided extracts suitable for microarray hybridization. Compared with Affymetrix One-Cycle labeled extracts, NuGEN system protocols utilizing oligo(dT) and random hexamer primers, and cDNA target preparations instead of cRNA, achieved percent present rates up to 55% on Plus 2.0 arrays. Based on two paired-sample analyses, at 90% specificity this equalled an average 30 percentage-point increase (from 50% to 80%) in FFPE transcript sensitivity relative to fresh frozen tissues, which we have assumed to have 100% sensitivity and specificity. The high content of Exon arrays, with multiple probe sets per exon, improved FFPE sensitivity to 92% at 96% specificity, corresponding to an absolute increase of ~600 genes over Plus 2.0 arrays. While larger series are needed to confirm high correspondence between fresh-frozen and FFPE expression patterns, these data suggest that both Plus 2.0 and Exon arrays are suitable platforms for FFPE microarray expression analyses.
2009-07-01T00:00:00Z