Topical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha.
Wolf, Peter Maier, Harald Müllegger, Robert R Chadwick, Caroline A Hofmann-Wellenhof, Rainer Soyer, H Peter Hofer, Angelika Smolle, Josef Horn, Michael Cerroni, Lorenzo
Wolf, Peter
Maier, Harald
Müllegger, Robert R
Chadwick, Caroline A
Hofmann-Wellenhof, Rainer
Soyer, H Peter
Hofer, Angelika
Smolle, Josef
Horn, Michael
Cerroni, Lorenzo
Citations
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Abstract
Exposing human skin to ultraviolet radiation causes DNA damage, sunburn, immune alterations, and eventually, skin cancer. We wished to determine whether liposomes containing a DNA repair enzyme could prevent any of the acute effects of irradiation when applied after ultraviolet exposure. Fifteen human patients with a prior history of skin cancer were exposed to two minimal erythema doses of ultraviolet radiation on their buttock skin. Liposomes containing T4 endonuclease V or heat-inactivated enzyme were applied immediately and at 2, 4, and 5 h after ultraviolet irradiation. Transmission electron microscopy after anti-T4 endonuclease V-staining and immunogold labeling on biopsies taken at 6 h after ultraviolet exposure revealed that the enzyme was present within cells in the skin. Immunohistochemical DNA damage studies suggested a trend toward improved DNA repair at the active T4 endonuclease V liposome-treated test sites. Although the active T4 endonuclease V liposomes did not significantly affect the ultraviolet-induced erythema response and microscopic sunburn cell formation, they nearly completely prevented ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha RNA message and of interleukin-10 protein. These studies demonstrate that liposomes can be used for topical intracellular delivery of small proteins to human skin and suggest that liposomes containing DNA repair enzymes may provide a new avenue for photoprotection against some forms of ultraviolet-induced skin damage.
Authors
Wolf, Peter
Maier, Harald
Müllegger, Robert R
Chadwick, Caroline A
Hofmann-Wellenhof, Rainer
Soyer, H Peter
Hofer, Angelika
Smolle, Josef
Horn, Michael
Cerroni, Lorenzo
Yarosh, Daniel
Klein, Jonathan
Bucana, Corazon
Dunner, Kenneth
Potten, Christopher S
Hönigsmann, Herbert
Kerl, Helmut
Kripke, Margaret L
Maier, Harald
Müllegger, Robert R
Chadwick, Caroline A
Hofmann-Wellenhof, Rainer
Soyer, H Peter
Hofer, Angelika
Smolle, Josef
Horn, Michael
Cerroni, Lorenzo
Yarosh, Daniel
Klein, Jonathan
Bucana, Corazon
Dunner, Kenneth
Potten, Christopher S
Hönigsmann, Herbert
Kerl, Helmut
Kripke, Margaret L
Description
Date
2000-01
Publisher
Collections
Keywords
Tumour Necrosis Factor-alpha
Type
Article
Citation
Topical treatment with liposomes containing T4 endonuclease V protects human skin in vivo from ultraviolet-induced upregulation of interleukin-10 and tumor necrosis factor-alpha. 2000, 114 (1):149-56 J. Invest. Dermatol.