Monoclonal antibodies to human embryonal carcinoma cells: antigenic relationships of germ cell tumors.
Rinke de Wit, T F Wilson, L Van den Elsen, P J Thielen, F Brekhoff, D Oosterhuis, J W Pera, M F Stern, Peter L
Rinke de Wit, T F
Wilson, L
Van den Elsen, P J
Thielen, F
Brekhoff, D
Oosterhuis, J W
Pera, M F
Stern, Peter L
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Abstract
Fifteen monoclonal antibodies (mAb) that show specificity for human embryonal carcinoma cells are described. C57BL/6 mice were immunized with Tera-2 embryonal carcinoma cells, and hybridomas were isolated and tested versus a set of human developmental tumor cell lines. The antigens exhibit relatively restricted and unique distributions on normal tissues as shown by immunohistochemistry. The mAbs recognize a series of differentiation antigens since their expression changes when teratocarcinoma stem cells are induced to differentiate in vitro. The expression of these molecules in germ cell and related tumors is consistent with the data obtained from in vitro cell studies. Seven of these mAbs immunoprecipitate molecules from surface labeled Tera-2 cells that show distinct molecular weights on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The antigenic phenotypes defined by the set of mAbs can be used to investigate possible pathogenetic relationships of the various testicular tumors.
Description
Date
1991-08
Publisher
Collections
Keywords
Cancer Antigens
Cancerous Stem Cells
Cultured Tumour Cells
Cancerous Stem Cells
Cultured Tumour Cells
Type
Article
Citation
Monoclonal antibodies to human embryonal carcinoma cells: antigenic relationships of germ cell tumors. 1991, 65 (2):180-91 Lab. Invest.