CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors.
De Wynter, Erika A Buck, D Hart, Claire A Heywood, R Coutinho, Lucia H Clayton, Alison J Rafferty, Joseph A Burt, Deborah J Guenechea, G Bueren, J A
De Wynter, Erika A
Buck, D
Hart, Claire A
Heywood, R
Coutinho, Lucia H
Clayton, Alison J
Rafferty, Joseph A
Burt, Deborah J
Guenechea, G
Bueren, J A
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Abstract
The AC133 antigen is a novel antigen selectively expressed on a subset of CD34+ cells in human fetal liver, bone marrow, and blood as demonstrated by flow cytometric analyses. In this study, we have further assessed the expression of AC133 on CD34+ cells in hemopoietic samples and found that there was a highly significant difference between normal bone marrow and cord blood versus aphereses (p <0.0001) but not between bone marrow and cord blood. Most of the clonogenic cells (67%) were contained in the CD34+AC133+ fraction. Compared with cultures of the CD34+AC133- cells, generation of progenitor cells in long-term culture on bone marrow stroma was consistently 10- to 100-fold higher in cultures initiated with CD34+AC133+ cells and was maintained for the 8-10 weeks of culture. Only the CD34+AC133+ cells were capable of repopulating NOD/SCID mice. Human cells were detectable as early as day 20, with increased levels (67%) apparent 40 days post-transplantation. Five thousand CD34+AC133+ cells engrafted about 20% of the mice, while no engraftment was observed in animals transplanted with up to 1.2 x 10(5) CD34+AC133- cells. The CD34+AC133+ population was also enriched (seven-fold) in dendritic cell precursors, and the dendritic cells generated were functionally active in a mixed lymphocyte reaction assay. AC133+ cells should be useful in the study of cellular and molecular mechanisms regulating primitive hemopoietic cells.
Description
Date
1998
Publisher
Collections
Keywords
Foetal Blood
Haematopoiesis
Haematopoietic Stem Cells
Haematopoiesis
Haematopoietic Stem Cells
Type
Article
Citation
CD34+AC133+ cells isolated from cord blood are highly enriched in long-term culture-initiating cells, NOD/SCID-repopulating cells and dendritic cell progenitors. 1998, 16 (6):387-96 Stem Cells