Plo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle.
Anderson, Mark Ng, Szu Shien Marchesi, Vanessa MacIver, Fiona H Stevens, Frances E Riddell, Tracy Glover, David M Hagan, Iain M McInerny, Christopher J
Anderson, Mark
Ng, Szu Shien
Marchesi, Vanessa
MacIver, Fiona H
Stevens, Frances E
Riddell, Tracy
Glover, David M
Hagan, Iain M
McInerny, Christopher J
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Abstract
The regulation of gene expression plays an important part in cell cycle controls. We describe the molecular machinery that co-ordinates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle. A sequence is identified in the cdc15(+) promoter that we call a PCB (pombe cell cycle box), which confers M-G(1)-specific transcription. Sequences similar to the PCB are present in the promoters of seven other genes, spo12(+), cdc19(+), fin1(+), sid2(+), ppb1(+), mid1(+)/dmf1(+) and plo1(+), which we find to be transcribed at M-G(1). A transcription factor complex is identified that binds to the PCB sequence, which we name PBF, for PCB-binding factor. Finally, we show that PBF binding activity and consequent gene transcription are regulated by the Plo1p protein kinase, thus invoking a potential auto-feedback loop mechanism that regulates mitotic gene transcription and passage through septation and cytokinesis.
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Date
2002-11-01
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Article
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Plo1(+) regulates gene transcription at the M-G(1) interval during the fission yeast mitotic cell cycle. 2002, 21 (21):5745-55 EMBO J.