Accessibility of chromatin to DNA polymerase I and location of the F1 histone.
Saffhill, Roy Itzhaki, Ruth F
Saffhill, Roy
Itzhaki, Ruth F
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Abstract
The effect of prebound poly-L-lysine upon the template activity of DNA, chromatin and F1 histone-depleted chromatin for E. coli DNA polymerase I has been investigated. Measurements have been made in the absence and presence of 0.1M NaCl. From the results we conclude that only ca 60% of the polylysine-accessible DNA, i.e. 22% of the total DNA of the chromatin, is accessible to the DNA polymerase I and that ca. 30% of the polylysine-accessible DNA, i.e. 11% of the total DNA, is associated with the F1 histone.
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1975-01
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Accessibility of chromatin to DNA polymerase I and location of the F1 histone. 1975, 2 (1):113-9 Nucleic Acids Res.