Expression of human CD4 by two human-mouse interlineage hybrids.
Taylor, G M Morten, J E Morten, H Dodge, A B Ridway, J C Jones, P M Harris, R
Taylor, G M
Morten, J E
Morten, H
Dodge, A B
Ridway, J C
Jones, P M
Harris, R
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Abstract
Two hybrid cell lines expressing human CD4 were prepared by fusing human B-lymphoid cells with the mouse T-lymphoma BW5147. Hybrid TF42 was derived from a human B-lymphoblastoid line and TF53.1 from a human B-ALL. Variants of these hybrids expressing or lacking CD4 were isolated by sorting cells stained with the monoclonal antibody (mAb) OKT4 on a fluorescence-activated cell sorter (FACS). Cytogenetic, isoenzyme and DNA analysis confirmed the presence of human chromosome 12 in the CD4+ hybrids, and revealed that CD4 expression by TF42 was associated with multiple copies of this chromosome. Of seventy mAb recognizing human T-cell antigens screened on the CD4+ and CD4- variants of the two hybrids, only mAb recognizing CD4 and Leu 8 reacted with the CD4+ cells. These hybrids should be useful in the preparation, screening and analysis of anti-CD4 monoclonal antibodies, and in studies of CD4 epitopes recognized by HIV.
Description
Date
1988-08
Publisher
Collections
Keywords
B-Cell Leukaemia
Cultured Tumour Cells
Cultured Tumour Cells
Type
Article
Citation
Expression of human CD4 by two human-mouse interlineage hybrids. 1988, 15 (4):197-208 J. Immunogenet.