Chemical heterogeneity of heparan sulfate from a human neuroblastoma cell line.
Fransson, L A Hampson, Ian N Kumar, Shant Gallagher, John T
Fransson, L A
Hampson, Ian N
Kumar, Shant
Gallagher, John T
Citations
Altmetric:
Abstract
The chemical heterogeneity of radiolabelled neuroblastoma heparan sulfate has been studied by ion exchange chromatography and by affinity chromatography on heparan sulfate-agarose. Although the entire population of chains shows considerable homogeneity in charge density, the deaminative cleavage products ranged in size from disaccharides to eicosasaccharides. Under appropriate conditions neuroblastoma heparan sulfate could be separated into two pools of low or high affinity for lung heparan sulfate-agarose. Analyses of periodate oxidation-alkaline elimination indicated that the high affinity chains contained larger proportions of heparin-like segments, i.e. iduronate-rich and N-sulfated ones.
Affiliation
Description
Date
1985
Publisher
Keywords
Type
Article
Citation
Chemical heterogeneity of heparan sulfate from a human neuroblastoma cell line. 1985, 39 (4):305-13 Acta Chem Scand, B, Org Chem Biochem