Detection of ras gene alterations and ras proteins in colorectal cancer.
Salhab, Nabil Jones, David J Bos, J L Kinsella, Anne R Schofield, Philip F
Salhab, Nabil
Jones, David J
Bos, J L
Kinsella, Anne R
Schofield, Philip F
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Abstract
DNA extracted from 31 primary colorectal carcinomas was analyzed for the presence of ras gene amplification and mutations. Nine carcinomas had Ha-ras amplification and seven Ki-ras amplification. Nine carcinomas had codon 12 Ki-ras mutations. Immunohistochemical staining for ras proteins revealed a normal membrane association in normal mucosa and benign polyps but an abnormal cytoplasmic distribution in carcinomas. Amplification, mutations, and immunohistochemical staining were independent of histologic differentiation, Dukes' stage, or DNA ploidy status. This study demonstrates that abnormalities of ras genes are a common finding in colorectal carcinomas. They are potentially important biologic changes associated with malignancy, although they do not appear to be related to clinical behavior.
Description
Date
1989-08
Publisher
Collections
Keywords
Colonic Cancer
Cancer DNA
Rectal Cancer
Cancer DNA
Rectal Cancer
Type
Article
Citation
Detection of ras gene alterations and ras proteins in colorectal cancer. 1989, 32 (8):659-64 Dis. Colon Rectum