Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle.
Tamm, T Grallert, Agnes Grossman, Emily P S Alvarez-Tabares, Isabel Stevens, Frances E Hagan, Iain M
Tamm, T
Grallert, Agnes
Grossman, Emily P S
Alvarez-Tabares, Isabel
Stevens, Frances E
Hagan, Iain M
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Abstract
The fission yeast interphase spindle pole body (SPB) is a bipartite structure in which a bulky cytoplasmic domain is separated from a nuclear component by the nuclear envelope. During mitosis, the SPB is incorporated into a fenestra that forms within the envelope during mitotic commitment. Closure of this fenestra during anaphase B/mitotic exit returns the cytoplasmic component to the cytoplasmic face of an intact interphase nuclear envelope. Here we show that Brr6 is transiently recruited to SPBs at both SPB insertion and extrusion. Brr6 is required for both SPB insertion and nuclear envelope integrity during anaphase B/mitotic exit. Genetic interactions with apq12 and defective sterol assimilation suggest that Brr6 may alter envelope composition at SPBs to promote SPB insertion and extrusion. The restriction of the Brr6 domain to eukaryotes that use a polar fenestra in an otherwise closed mitosis suggests a conserved role in fenestration to enable a single microtubule organizing center to nucleate both cytoplasmic and nuclear microtubules on opposing sides of the nuclear envelope.
Authors
Tamm, T
Grallert, Agnes
Grossman, Emily P S
Alvarez-Tabares, Isabel
Stevens, Frances E
Hagan, Iain M
Grallert, Agnes
Grossman, Emily P S
Alvarez-Tabares, Isabel
Stevens, Frances E
Hagan, Iain M
Affiliation
Cancer Research UK Cell Division Group, Paterson Institute for Cancer Research, Manchester M20 4BX, England, UK.
Description
Date
2011-10-31
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Collections
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Article
Citation
Brr6 drives the Schizosaccharomyces pombe spindle pole body nuclear envelope insertion/extrusion cycle. 2011, 195 (3):467-84 J Cell Biol