Delayed-type hypersensitivity response to human papillomavirus type 16 E6 protein in a mouse model.
Chambers, M A Stacey, Simon N Arrand, John R Stanley, M A
Chambers, M A
Stacey, Simon N
Arrand, John R
Stanley, M A
Citations
Altmetric:
Abstract
A mouse model incorporating the epitheliotropic nature of human papillomavirus (HPV) infections has been used to study an immune response to HPV type 16 (HPV-16) E6 protein in vivo. Using a transplantation technique, a novel immortal keratinocyte cell line expressing the E6 protein has been grafted onto syngeneic mice to re-form a differentiated epithelium overlying a granulation tissue bed. By this approach the presentation of viral antigens to the immune system can be modelled in a way analogous to the natural infection. Here we report a delayed-type hypersensitivity (DTH) reaction in grafted mice challenged intradermally with a recombinant vaccinia virus expressing the HPV-16 E6 protein. The specificity of the response was confirmed by the absence of a DTH reaction to challenge with virus expressing either HPV-16 E7 or L1 protein.
Description
Date
1994-01
Publisher
Collections
Keywords
Type
Article
Citation
Delayed-type hypersensitivity response to human papillomavirus type 16 E6 protein in a mouse model. 1994, 75 ( Pt 1):165-9 J. Gen. Virol.