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Preparation of protein extracts from Schizosaccharomyces pombe using trichloroacetic acid precipitation.

Grallert, Agnes
; 
Hagan, Iain M
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Abstract
Schizosaccharomyces pombe is an attractive model organism with which to study core principles of conserved molecular cell biology processes. The ability to monitor protein behavior following separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) underpins much of this activity. Here we describe a robust protocol for the preparation of protein samples for analysis by SDS-PAGE.
Authors
Grallert, Agnes
Hagan, Iain M
Affiliation
Agnes.Grallert@cruk.manchester.ac.uk Iain.Hagan@cruk.manchester.ac.ukCRUK Cell Division Group, Cancer Research UK Manchester Institute, University of Manchester, Manchester M20 4BX, United Kingdom
Description
Date
2017-02-01
Publisher
Collections
All Paterson Institute for Cancer Research
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Type
Article
Citation
Preparation of protein extracts from Schizosaccharomyces pombe using trichloroacetic acid precipitation. 2017, Cold Spring Harb Protoc
Journal Title
Journal ISSN
Volume Title
URI
http://hdl.handle.net/10541/620187
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