Multilineage gene expression precedes commitment in the hemopoietic system.
Hu, M Krause, D Greaves, M Sharkis, S Dexter, T Michael Heyworth, Clare M Enver, T
Hu, M
Krause, D
Greaves, M
Sharkis, S
Dexter, T Michael
Heyworth, Clare M
Enver, T
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Abstract
We have tested the hypothesis that multipotential hemopoietic stem and progenitor cells prime several different lineage-affiliated programs of gene activity prior to unilineage commitment and differentiation. Using single cell RT-PCR we show that erythroid (beta-globin) and myeloid (myeloperoxidase) gene expression programs can be initiated by the same cell prior to exclusive commitment to the erythroid or granulocytic lineages. Furthermore, the multipotential state is characterized by the coexpression of several lineage-affiliated cytokine receptors. These data support a model of hemopoietic lineage specification in which unilineage commitment is prefaced by a "promiscuous" phase of multilineage locus activation.
Description
Date
1997-03-15
Publisher
Collections
Keywords
Haematopoiesis
Haematopoietic Stem Cells
Haematopoietic Stem Cells
Type
Article
Citation
Multilineage gene expression precedes commitment in the hemopoietic system. 1997, 11 (6):774-85 Genes Dev.