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Nuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP).
El Bounkari, Omar Guria, Anuja Klebba-Faerber, Sabine Claussen, Maike Pieler, Tomas Griffiths, John R Whetton, Anthony D Koch, Alexandra Tamura, Teruko
El Bounkari, Omar
Guria, Anuja
Klebba-Faerber, Sabine
Claussen, Maike
Pieler, Tomas
Griffiths, John R
Whetton, Anthony D
Koch, Alexandra
Tamura, Teruko
Citations
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Abstract
THOC7 and Fms-interacting protein (FMIP) are members of the THO complex that associate with the mRNA export apparatus. FMIP is a nucleocytoplasmic shuttling protein with a nuclear localization signal (NLS), whereas THOC7 does not contain a typical NLS motif. We show here that THOC7 (50-137, amino acid numbers) binds to the N-terminal portion (1-199) of FMIP directly. FMIP is detected mainly in the nucleus. In the absence of exogenous FMIP, THOC7 resides mainly in the cytoplasm, while in the presence of FMIP, THOC7 is transported into the nucleus with FMIP. Furthermore, THOC7 lacking the FMIP binding site does not co-localize with FMIP, indicating that THOC7/FMIP interaction is required for nuclear localization of THOC7.
Description
Date
2009-01-05
Publisher
Keywords
mRNA
THOC7
FMIP
Fms Tyrosine Kinase Interacting Protein
THOC7
FMIP
Fms Tyrosine Kinase Interacting Protein
Type
Article
Citation
Nuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP). 2009, 583 (1):13-8 FEBS Lett.