Optical artefacts in transflection mode FTIR microspectroscopic images of single cells on a biological support: the effect of back-scattering into collection optics
Lee, Joe Gazi, Ehsan Dwyer, John Brown, Michael D Nicholson, James M Gardner, Peter
Lee, Joe
Gazi, Ehsan
Dwyer, John
Brown, Michael D
Nicholson, James M
Gardner, Peter
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Abstract
Infrared microspectroscopic imaging data of single human prostate cancer cells, on an artificial extracellular matrix (Matrigel) thin-film surface, are presented. The spectral intensity maps, obtained in reflection mode, appear to show that the protein intensity distribution observed at the location of a cell changes dramatically depending on the concentration and/or thickness of the underlying Matrigel layer. Specifically, cells adhered to a low protein concentration or thin surface exhibit a higher protein intensity signal than the surrounding layer whereas those on a high protein concentration or thick surface exhibit a lower protein intensity signal. These results are qualitatively explained by a simple model that takes into account the fact that radiation scattered from cells can enter the collection optics of the microscope without passing through the Matrigel layer. This leads to an apparent reduction in absorption at the cell.
Description
Date
2007-08
Publisher
Collections
Keywords
Cell Line Tumour
Prostate Cancer
Prostate Cancer
Type
Article
Citation
Optical artefacts in transflection mode FTIR microspectroscopic images of single cells on a biological support: the effect of back-scattering into collection optics. 2007, 132 (8):750-5 Analyst