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PAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN.
Li, Hong Gui Wang, Qiuyu Hong, Mei Li Kumar, Shant Parker, Craig Slevin, Mark Kumar, Patricia
Li, Hong Gui
Wang, Qiuyu
Hong, Mei Li
Kumar, Shant
Parker, Craig
Slevin, Mark
Kumar, Patricia
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Abstract
PAX3 or PAX3-FKHR expression is implicated in cell transformation and tumourigenesis. Here, C2C12 myoblasts were transfected with a sense Pax3 vector and a pTet-On system to induce Pax3 expression, whereas to downregulate PAX3-FKHR, Rh18 was transfected with an antisense Pax3 with a pTet-On system. The inhibition of PAX3-FKHR in Rh18 induced upregulation of PTEN. Decreased resistance to apoptosis and increased transformation ability were observed in the Rh18 cells with PAX3-FKHR downregulation. Conversely, Pax3 induction in C2C12 cells downregulated the expression of PTEN and p27(Kip1). These results indicate that the involvement of PAX3 and PAX3-FKHR in rhabdomyosarcoma tumourigenesis may be through downregulation of PTEN tumour suppressor gene, affecting the PTEN/AKT survival pathway.
Description
Date
2007-08-18
Publisher
Collections
Keywords
Cell Line Tumour
Muscle Cancer
Muscle Cancer
Type
Article
Citation
PAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN. 2007, 253 (2):215-23 Cancer Lett.