Direct evidence of lipid translocation between adipocytes and prostate cancer cells with imaging FTIR microspectroscopy.
Gazi, Ehsan Gardner, Peter Lockyer, Nicholas P Hart, Claire A Brown, Michael D Clarke, Noel W
Gazi, Ehsan
Gardner, Peter
Lockyer, Nicholas P
Hart, Claire A
Brown, Michael D
Clarke, Noel W
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Abstract
Various epidemiological studies show a positive correlation between high intake of dietary FAs and metastatic prostate cancer (CaP). Moreover, CaP metastasizes to the bone marrow, which harbors a rich source of lipids stored within adipocytes. Here, we use Fourier transform infrared (FTIR) microspectroscopy to study adipocyte biochemistry and to demonstrate that PC-3 cells uptake isotopically labeled FA [deuterated palmitic acid (D(31)-PA)] from an adipocyte. Using this vibrational spectroscopic technique, we detected subcellular locations in a single adipocyte enriched with D(31)-PA using the upsilon(as+s)(C-D)(2+3) (D(31)-PA): upsilon(as+s)(C-H)(2+3) (lipid hydrocarbon) signal. In addition, larger adipocytes were found to consist of a higher percentage of D(31)-PA of the total lipid found within the adipocyte. Following background subtraction, the upsilon(as)(C-D)(2+3) signal illuminated starved PC-3 cells cocultured with D(31)-PA-loaded adipocytes, indicating translocation of the labeled FA. This study demonstrates lipid-specific translocation between adipocytes and tumor cells and the use of FTIR microspectroscopy to characterize various biomolecular features of a single adipocyte without the requirement for cell isolation and lipid extraction.
Description
Date
2007-08
Publisher
Keywords
Prostatic Cancer
Tumour Cells
Tumour Markers
Tumour Cells
Tumour Markers
Type
Article
Citation
Direct evidence of lipid translocation between adipocytes and prostate cancer cells with imaging FTIR microspectroscopy. 2007, 48 (8):1846-56 J. Lipid Res.