Protocol to study the inheritance and propagation of non-genetically encoded states using barcode decay lineage tracing
Shlyakhtina, Yelyzaveta Bloechl, Bloechl Moran, Katherine L Portal, Maximiliano M
Shlyakhtina, Yelyzaveta
Bloechl, Bloechl
Moran, Katherine L
Portal, Maximiliano M
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Abstract
Here, we present a protocol to perform barcode decay lineage tracing followed by single-cell transcriptome analysis (BdLT-Seq). We describe steps for BdLT-Seq experimental design, building barcoded episome reporters, performing episome transfection, and barcode retrieval. We then describe procedures for sequencing library construction while providing options for sample multiplexing and data analysis. This BdLT-Seq technique enables the assessment of clonal evolution in a directional manner while preserving isogeneity, thus allowing the comparison of non-genetic molecular features between isogenic cell lineages. For complete details on the use and execution of this protocol, please refer to Shlyakhtina et al. (2023).(1).
Description
Date
2024
Publisher
Collections
Keywords
Type
Protocol
Citation
Shlyakhtina Y, Bloechl B, Moran KL, Portal MM. Protocol to study the inheritance and propagation of non-genetically encoded states using barcode decay lineage tracing. STAR protocols. 2024 Jan 4;5(1):102809. PubMed PMID: 38180835. Pubmed Central PMCID: 10801334.