Association of radiomic features with aggressive phenotypes in soft tissue sarcomas
Datta, Anubhav Forker, Laura-Jane McWilliam, Alan Mistry, Hitesh Zhong, J. Wylie, James P Coyle, Catherine Saunders, Daniel Kennedy, S. O'Connor, James P B
Datta, Anubhav
Forker, Laura-Jane
McWilliam, Alan
Mistry, Hitesh
Zhong, J.
Wylie, James P
Coyle, Catherine
Saunders, Daniel
Kennedy, S.
O'Connor, James P B
Citations
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Abstract
Purpose or Objective
Soft tissue sarcomas(STS) are rare and heterogeneous tumours with variable outcomes. Improving survival
requires identifying and targeting aggressive phenotypes. Novel ways of stratifying patients include a clinical
prognostic nomogram(Sarculator; includes tumour size) and a gene expression derived hypoxia score(HS). We
investigate the feasibility of non-invasive and repeatable imaging to assess clinically/biologically relevant and
targetable phenotypes.
Materials and Methods
Retrospective analysis of 43 extremity STS patients with matched diagnostic biopsy-imaging data was
performed. Imaging was acquired at several different hospitals in the region using various scanners/protocols.
Patients underwent curative-intent surgery±adjuvant radiotherapy. HS(24-gene signature) were measured
using NanoString. Sarculator predicted 10yr OS.
Treatment naïve T1(n=41) and T2(n=28) weighted sequences were segmented by two radiologists using
Raystation. Histogram normalisation and gray-level intensity discretisation steps were performed. PyRadiomics
v3.0.1 was used to extract features and robustness was assessed using intra-class correlation(ICC; threshold
>0.9). Features with a high degree of association within their classes were further selected using Spearman’s
rank correlation. Associations with Sarculator and HS were determined using rank correlation matrices and
principal component analysis(PCA). Significance levels were set at p<0.05.
Results
ICC identified 63(T1) and 68(T2) features. Further selection resulted in 4(T1) and 14(T2) exploratory features.
Sarculator correlated strongly with T1(ρ=-0.75) and T2(ρ=-0.84) volume features (Fig 1). T1 size(ρ=0.44)
correlated strongest with HS. Top T2 features, gray-level non-uniformity(GLN) and zone entropy(ZE),
correlated with Sarculator(ρ=-0.57,ρ=-0.56 respectively) and with hypoxia(ρ=-0.37,ρ=0.39 respectively). GLN
is a gray-level run length matrix (GLRLM) feature quantifying variability of gray-level intensity. ZE is a gray-level size zone matrix feature quantifying randomness in distribution zone sizes and gray levels. High GLN
values indicate more heterogeneity in intensity; high ZE values indicate more heterogeneity in texture. PCA
identified clusters using the patient radiomics values, and box plots highlight differences (Fig 2). T1 derived
features were significantly different between the 3 groups for Sarculator(p=0.013) but not HS(p=0.156). There
were no significant differences identified by T2 derived features for Sarculator(p=0.088) or HS(p=0.676). Conclusion
Shape-related T1- and T2- MRI derived radiomics features of STS correlated with Sarculator but less well with
HS. The T1 radiomic values differentiated patient groups with different Sarculator scores, suggesting potential
to non-invasively identify aggressive STS phenotypes. Radiomic profiling of STS is feasible and further study is
worthwhile.
Description
Date
2021
Publisher
Collections
Keywords
Type
Meetings and Proceedings
Citation
Datta A, Forker L, McWilliam A, Mistry H, Zhong J, Wylie J, et al. Association of radiomic features with aggressive phenotypes in soft tissue sarcomas. Radiotherapy and Oncology. 2021;161:S1162-S3.