Ovarian tissue harvested from lymphoma patients to preserve fertility may be safe for autotransplantation.
Kim, S Samuel Radford, John A Harris, Martin Varley, Jennifer Rutherford, Anthony J Lieberman, Brian A Shalet, Stephen M Gosden, Roger
Kim, S Samuel
Radford, John A
Harris, Martin
Varley, Jennifer
Rutherford, Anthony J
Lieberman, Brian A
Shalet, Stephen M
Gosden, Roger
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Abstract
BACKGROUND: Ovarian failure is a common sequel to chemo/radiotherapy in patients successfully treated for cancer. Harvesting, cryopreserving and subsequently re-implanting ovarian cortical grafts can be used to re-establish reproductive potential in women with cancer. The safety issue, however, is of great concern because residual disease in autografted ovarian tissues might cause recrudescence of disease. METHODS: A total of 30 non-obese diabetic severe combined immunodeficient (NOD/LtSz-SCID) mice were individually xenografted s.c. with frozen-thawed ovarian tissue from 18 patients with lymphoma [13 Hodgkin's lymphoma (HL) and 5 non-Hodgkin's lymphoma (NHL)]. The animals were autopsied at 16 weeks, or earlier if cachectic. The xenograft, liver, spleen, sternum, para-aortic lymph nodes and thymus were prepared for histology, immunohistochemistry and human DNA microsatellite analysis. RESULTS: None of the animals grafted with ovarian tissue from lymphoma patients developed disease. However, all 3 animals grafted with lymph node tissue from an NHL patient developed B-cell lymphomas that were confirmed as human in origin by DNA microsatellite analysis. CONCLUSION: Ovarian tissue harvested before high-dose chemotherapy for HL or NHL may not carry a risk of disease transmission by autotransplantation, although the possibility is difficult to exclude completely.
Description
Date
2001-10
Publisher
Keywords
Cancer Invasiveness
Type
Article
Citation
Ovarian tissue harvested from lymphoma patients to preserve fertility may be safe for autotransplantation. 2001, 16 (10):2056-60 Hum. Reprod.