Evaluation and validation of a robust single cell RNA-amplification protocol through transcriptional profiling of enriched lung cancer initiating cells.
Rothwell, Dominic G Li, Yaoyong Ayub, Mahmood Tate, Catriona Newton, Gillian Hey, Yvonne Carter, Louise Faulkner, Suzanne Moro, Massimo Pepper, Stuart D
Rothwell, Dominic G
Li, Yaoyong
Ayub, Mahmood
Tate, Catriona
Newton, Gillian
Hey, Yvonne
Carter, Louise
Faulkner, Suzanne
Moro, Massimo
Pepper, Stuart D
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Abstract
Although profiling of RNA in single cells has broadened our understanding of development, cancer biology and mechanisms of disease dissemination, it requires the development of reliable and flexible methods. Here we demonstrate that the EpiStem RNA-Amp™ methodology reproducibly generates microgram amounts of cDNA suitable for RNA-Seq, RT-qPCR arrays and Microarray analysis.
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Date
2014
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Collections
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Article
Citation
Evaluation and validation of a robust single cell RNA-amplification protocol through transcriptional profiling of enriched lung cancer initiating cells. 2014, 15:1129 BMC Genomics