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ATM-dependent phosphorylation of ATF2 is required for the DNA damage response.
Bhoumik, Anindita Takahashi, Shoichi Shiloh, Yosef Jones, Nic Ronai, Ze'ev Breitwieser, Wolfgang
Bhoumik, Anindita
Takahashi, Shoichi
Shiloh, Yosef
Jones, Nic
Ronai, Ze'ev
Breitwieser, Wolfgang
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Abstract
Activating transcription factor 2 (ATF2) is regulated by JNK/p38 in response to stress. Here, we demonstrate that the protein kinase ATM phosphorylates ATF2 on serines 490 and 498 following ionizing radiation (IR). Phosphoantibodies to ATF2(490/8) reveal dose- and time-dependent phosphorylation of ATF2 by ATM that results in its rapid colocalization with gamma-H2AX and MRN components into IR-induced foci (IRIF). Inhibition of ATF2 expression decreased recruitment of Mre11 to IRIF, abrogated S phase checkpoint, reduced activation of ATM, Chk1, and Chk2, and impaired radioresistance. ATF2 requires neither JNK/p38 nor its DNA binding domain for recruitment to IRIF and the S phase checkpoint. Our findings identify a role for ATF2 in the DNA damage response that is uncoupled from its transcriptional activity.
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Date
2005-05-27
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Article
Citation
ATM-dependent phosphorylation of ATF2 is required for the DNA damage response. 2005, 18 (5):577-87 Mol. Cell