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Segregation of genomes in polyploid tumour cells following mitotic catastrophe.
Erenpreisa, Jekaterina Kalejs, Martins Ianzini, Fiorenza Kosmacek, Elizabeth A Mackey, Mike Emzinsh, Dzintars Cragg, Mark S Ivanov, Andrei
Erenpreisa, Jekaterina
Kalejs, Martins
Ianzini, Fiorenza
Kosmacek, Elizabeth A
Mackey, Mike
Emzinsh, Dzintars
Cragg, Mark S
Ivanov, Andrei
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Abstract
Following irradiation p53-function-deficient tumour cells undergo mitotic catastrophe and form endopolyploid cells. A small proportion of these segregates nuclei, and give rise to viable descendants. Here we studied this process in five tumour cell lines. After mitotic failure, tumour cells enter the endocycle and form mono-nucleated or multi-nucleated giant cells (MOGC and MNGC). MNGC arise from arrested anaphases, MOGC, from arrested metaphases. In both cases the individual genomes establish a radial pattern by links to a single microtubule organizing centre. Segregation of genomes is also ordered. MNGC present features of mitosis being resumed from late anaphase. In MOGC the sub-nuclei retain arrangement of stacked metaphase plates and are separated by folds of the nuclear envelope. Mitosis then resumes in sub-nuclei directly from metaphase. The data presented indicate that endopolyploid tumour cells preserve the integrity of individual genomes and can potentially re-initiate mitosis from the point at which it was interrupted.
Description
Date
2005-12
Publisher
Collections
Keywords
Cell Line Tumour
Tumour Suppressor Protein p53
Tumour Suppressor Protein p53
Type
Article
Citation
Segregation of genomes in polyploid tumour cells following mitotic catastrophe. 2005, 29 (12):1005-11 Cell Biol. Int.