Stimulator of proliferation of spleen colony-forming cells in acute sterile inflammation.
Milenković, P Ivanović, Z Lukić, M L Kataranovski, M Lord, Brian I
Milenković, P
Ivanović, Z
Lukić, M L
Kataranovski, M
Lord, Brian I
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Abstract
The presence and activity of a spleen colony-forming cell (CFU-S) proliferation stimulator was investigated in rat bone marrow after induction of sterile inflammation. Wistar rats were treated intraperitoneally with two 15 ml injections of 3.5% polyvinylpyrrolidone (PVP) at 18 h intervals, and the presence of CFU-S proliferation stimulator determined in bone marrow 6, 20 and 24 h after the first injection. The marrow of these mice was used to condition medium which was then fractionated using Amicon Diaflo ultrafiltration membranes. The 30-50 kDa fraction, taken from 20h post-PVP-bone marrow extract, was found to induce cycling of d8-CFU-S in normal mouse bone marrow. This activity was not related to the presence of interleukins-1, -2 or -6 like activities in the material tested. The results demonstrate the existence of CFU-S proliferation stimulator in the bone marrow of rats with sterile inflammation (i.e. in an in vivo tissue response to non-specific cell stimulation), similar to that originally described as a macrophage product in mouse bone marrow after treatment with a variety of cytotoxic agents.
Description
Date
1993-11
Publisher
Collections
Keywords
Haematopoietic Stem Cells
Type
Article
Citation
Stimulator of proliferation of spleen colony-forming cells in acute sterile inflammation. 1993, 26 (6):503-10 Cell Prolif.