Elementary protein analysis in schizosaccharomyces pombe.
Grallert, Agnes Hagan, Iain M
Grallert, Agnes
Hagan, Iain M
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Abstract
Biochemical monitoring and interrogation of protein function is a critical component of most fission yeast studies. In particular, its small proteome size, high conservation of core molecular cell biology, and genetic malleability make Schizosaccharomyces pombe an excellent model organism in which to use mass spectrometry to conduct proteome-wide approaches. Here we discuss issues encountered during the analysis of fission yeast protein preparations.
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2017-03-01
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Elementary protein analysis in schizosaccharomyces pombe. 2017, 2017 (3): Cold Spring Harb Protoc