An evaluation of gemcitabines differential radiosensitising effect in related bladder cancer cell lines.
Sangar, Vijay K Cowan, Richard A Margison, Geoffrey P Hendry, Jolyon H Clarke, Noel W
Sangar, Vijay K
Cowan, Richard A
Margison, Geoffrey P
Hendry, Jolyon H
Clarke, Noel W
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Abstract
The aim of this study was to establish the radiosensitising properties of gemcitabine in a pair of related bladder tumour cell lines with differential radiosensitivity. The radioresistant bladder tumour cell line MGH-U1 and its radiosensitive mutant clone, S40b (both p53 mutant), had SF(2) values (surviving fraction at 2 Gy) of 0.98 and 0.64, respectively (P<0.001). Colony-forming assays showed that at 0.01 microM gemcitabine radiosensitisation occurred only in the S40b cell line (dose-modifying factor (DMF)=1.4). At 0.3 microM (killing 50% of cells), both cell lines were radiosensitised; DMF=2.25 and 1.2 for MGH-U1 and S40b, respectively. These data suggest that gemcitabine is an effective radiosensitiser in bladder cancer cell lines, with greater sensitisation in the radioresistant parental line-a feature that should be useful in a clinical setting.
Description
Date
2004-01-26
Publisher
Keywords
Urinary Bladder Cancer
Cultured Tumour Cells
Cultured Tumour Cells
Type
Article
Citation
An evaluation of gemcitabines differential radiosensitising effect in related bladder cancer cell lines. 2004, 90 (2):542-8 Br. J. Cancer