Identification of a serpin specifically expressed in multipotent and bipotent hematopoietic progenitor cells and in activated T cells.
Hampson, Ian N Hampson, Lynne Pinkoski, M Cross, M Heyworth, Clare M Bleackley, R C Atkinson, E Dexter, T Michael
Hampson, Ian N
Hampson, Lynne
Pinkoski, M
Cross, M
Heyworth, Clare M
Bleackley, R C
Atkinson, E
Dexter, T Michael
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Abstract
We have identified a gene that has a high level of mRNA expression in undifferentiated, multipotential hematopoietic cells (FDCP-Mix) and that downregulates both transcript and protein, as these cells are induced to differentiate into mature myeloid cells. Sequence analysis of this gene has identified it as a serine protease inhibitor EB22/3 (serpin 2A). Constitutive expression of serpin 2A in FDCP-Mix cells was associated with an increase in the clonogenic potential of the cells and with a delay in the appearance of fully mature cells in cultures undergoing granulocyte macrophage differentiation when compared with control cells. Serpin 2A was also found to be expressed in bone marrow-derived bipotent granulocyte macrophage progenitor cells (GM-colony forming cell [CFC]), but not in erythrocyte progenitor cells from day 15 fetal liver. Expression of serpin 2A also showed a marked up regulation during the activation of cytotoxic suppressor CD8+ T cells, with a clear lag between the appearance of transcript and detection of protein.
Description
Date
1997-01-01
Publisher
Collections
Keywords
Haematopoietic Stem Cells
Type
Article
Citation
Identification of a serpin specifically expressed in multipotent and bipotent hematopoietic progenitor cells and in activated T cells. 1997, 89 (1):108-18 Blood