Early response gene signalling cascades activated by ionising radiation in primary human B cells.
Wilson, R E Taylor, S L Atherton, Graham T Johnston, D Waters, C M Norton, John D
Wilson, R E
Taylor, S L
Atherton, Graham T
Johnston, D
Waters, C M
Norton, John D
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Abstract
We have used a panel of 13 protein kinase C-responsive immediate early gene probes to dissect the cellular signalling pathways activated by ionising gamma radiation in primary human B cells. Of these 13 genes, a delayed transient induction was observed for only 8: c-fos, c-jun, jun-B, jun-D, c-myc, ergI/krox 24 and two 'anonymous' genes, 3L3 and 19A. Expression of c-myc and c-fos mRNAs was paralleled by the appearance of their encoded proteins suggesting that these oncoproteins may couple radiation signalling to cellular responses. Of three protein kinase C-coupled transcription factors examined by gel retardation assay, (AP1, NF kappa B, EgrK/Krox24) only NF kappa B and, to a lesser extent, AP1 was stimulated in response to irradiation. These observations are not obviously compatible with a simple model invoking protein kinase C in radiation signalling in primary B cells and suggest that the pleiotropic effects of ionising radiation on this cell type are mediated through a distinct cellular signalling cascade.
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Date
1993-12
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Article
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Early response gene signalling cascades activated by ionising radiation in primary human B cells. 1993, 8 (12):3229-37 Oncogene