Increases in c-Src expression level and activity do not promote the growth of human colorectal carcinoma cells in vitro and in vivo.
Welman, Arkadiusz Cawthorne, Christopher Ponce-Perez, Lourdes Barraclough, Jane Danson, Sarah Murray, Stephen M Cummings, Jeffrey Allen, Terence D Dive, Caroline
Welman, Arkadiusz
Cawthorne, Christopher
Ponce-Perez, Lourdes
Barraclough, Jane
Danson, Sarah
Murray, Stephen M
Cummings, Jeffrey
Allen, Terence D
Dive, Caroline
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Abstract
The levels and activity of c-Src in colorectal cancer cells increase steadily during the course of colorectal carcinogenesis and are most highly elevated in advanced metastatic disease. However, the effects of increases in c-Src activity on the proliferation of colorectal cancer cells during early and late stages of tumorigenesis remain elusive. To study the consequences of increases in c-Src levels and activity on the growth of colorectal cancer cells in later stages of colorectal carcinogenesis, we developed human colorectal cancer cell lines in which c-Src levels and activity could be inducibly increased by a tightly controlled expression of wild-type c-Src or of the constitutively active mutant of c-Src, c-SrcY527F. Src induction activated multiple signaling pathways (often associated with a proliferative response) but promoted neither cell proliferation in vitro nor tumor growth in a xenograft model in vivo. These results indicate that, in more advanced stages of colorectal carcinogenesis, increases in c-Src levels and activity are likely to have functions other than the direct promotion of tumor growth.
Description
Date
2006-11
Publisher
Collections
Keywords
Cell Line Tumour
Colorectal Cancer
Cancer Transplantation
Colorectal Cancer
Cancer Transplantation
Type
Article
Citation
Increases in c-Src expression level and activity do not promote the growth of human colorectal carcinoma cells in vitro and in vivo. 2006, 8 (11):905-16 Neoplasia