Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy.
Bassan, P Sachdeva, A Shanks, Jonathan H Brown, Michael D Gardner, P
Bassan, P
Sachdeva, A
Shanks, Jonathan H
Brown, Michael D
Gardner, P
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Abstract
FTIR chemical imaging has been demonstrated as a promising technique to construct automated systems to complement histopathological evaluation of biomedical tissue samples. The rapid chemical imaging of large areas of tissue has previously been a limiting factor in this application. Consequently, smaller areas of tissue have previously had to be sampled, possibly introducing sampling bias and potentially missing diagnostically important areas. In this report a high spatial resolution chemical image of a whole prostate cross section is shown comprising 66 million pixels. Each pixel represents an area 5.5 × 5.5 μm(2) of tissue and contains a full infrared spectrum providing a chemical fingerprint. The data acquisition time was 14 hours, thus showing that a clinical time frame of hours rather than days has been achieved.
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2013-12-07
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Whole organ cross-section chemical imaging using label-free mega-mosaic FTIR microscopy. 2013, 138 (23):7066-9 Analyst