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Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada.
Angelis, K Bríza, J Satava, J Skákal, I Velemínský, J Vlasák, J Kleibl, K Margison, Geoffrey P
Angelis, K
Bríza, J
Satava, J
Skákal, I
Velemínský, J
Vlasák, J
Kleibl, K
Margison, Geoffrey P
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Abstract
The protein coding region of the E. coli gene ada has been transferred to tobacco plants by a leaf disc transformation procedure involving an Agrobacterium tumefaciens Ti plasmid. Transformed plants were shown to be transgenic for the ada message and had increased levels of O6-alkylguanine DNA alkyltransferase activity. The N-methyl-N-nitrosourea- or taurinechlorethylnitrosourea-induced inhibition of growth of calluses or of cells in suspension was considerably lower in ada-transformed than in non-transformed plants. This indicates that O6-alkylguanine, O4-alkylthymine or phosphotriesters are growth-inhibitory lesions in tobacco.
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1992-05
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Increased resistance to the toxic effects of alkylating agents in tobacco expressing the E. coli DNA repair gene ada. 1992, 273 (3):271-80 Mutat. Res.