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Functional comparison of annexin V analogues labeled indirectly and directly with iodine-124.
Dekker, Bronwen A Keen, Heather G Shaw, David M Disley, Lynn Hastings, David L Hadfield, John A Reader, Andrew J Allan, Donald Julyan, Peter J Watson, Alastair
Dekker, Bronwen A
Keen, Heather G
Shaw, David M
Disley, Lynn
Hastings, David L
Hadfield, John A
Reader, Andrew J
Allan, Donald
Julyan, Peter J
Watson, Alastair
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Abstract
We are interested in imaging cell death in vivo using annexin V radiolabeled with (124)I. In this study, [(124)I]4IB-annexin V and [(124)I]4IB-ovalbumin were made using [(124)I]N-hydroxysuccinimidyl-4-iodobenzoate prepared by iododestannylation of N-hydroxysuccinimidyl-4-(tributylstannyl)benzoate. [(124)I]4IB-annexin V binds to phosphatidylserine-coated microtiter plates and apoptotic Jurkat cells and accumulates in hepatic apoptotic lesions in mice treated with anti-Fas antibody, while [(124)I]4IB-ovalbumin does not. In comparison with (124)I-annexin V, [(124)I]4IB-annexin V has a higher rate of binding to phosphatidylserine in vitro, a higher kidney and urine uptake, a lower thyroid and stomach content uptake, greater plasma stability and a lower rate of plasma clearance. Binding of radioactivity to apoptotic cells relative to normal cells in vitro and in vivo appears to be lower for [(124)I]4IB-annexin V than for (124)I-annexin V.
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Date
2005-05
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Article
Citation
Functional comparison of annexin V analogues labeled indirectly and directly with iodine-124. 2005, 32 (4):403-13 Nucl. Med. Biol.