In situ hybridisation and S1 mapping show that the presence of infiltrating plasma cells is associated with poor prognosis in breast cancer.
Parkes, H Collis, P Baildam, Andrew D Ralphs, D Lyons, B Howell, Anthony Craig, R
Parkes, H
Collis, P
Baildam, Andrew D
Ralphs, D
Lyons, B
Howell, Anthony
Craig, R
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Abstract
In order to identify potential markers of prognosis in breast cancer, representative cDNA libraries were constructed using RNA isolated from primary breast tumour tissue associated with good and poor prognosis. Cross-screening of these libraries repeatedly identified cloned mRNA species associated with the immune system, in particular B-cells, in libraries derived from tumours of poor prognosis. We have used one of these a kappa IV light chain cDNA probe, in two complementary studies to investigate the relationship between immunoglobin gene expression and prognosis. The results obtained using a combination of S1 mapping, RNA blotting and in situ hybridisation demonstrate that the presence of plasma cells, as defined by infiltrating cells which express high levels of immunoglobulin kappa-chain mRNA, is associated with a poor prognosis.
Description
Date
1988-12
Publisher
Collections
Keywords
Breast Cancer
Cancer DNA
Cancer RNA
Biological Tumour Markers
Cancer DNA
Cancer RNA
Biological Tumour Markers
Type
Article
Citation
In situ hybridisation and S1 mapping show that the presence of infiltrating plasma cells is associated with poor prognosis in breast cancer. 1988, 58 (6):715-22 Br J Cancer