A novel approach to the analysis of SUMOylation with the independent use of trypsin and elastase digestion followed by database searching utilising consecutive residue addition to lysine.
Chicooree, Navin Griffiths, John R Connolly, Yvonne Tan, Chong-Teik Malliri, Angeliki Eyers, C Smith, Duncan L
Chicooree, Navin
Griffiths, John R
Connolly, Yvonne
Tan, Chong-Teik
Malliri, Angeliki
Eyers, C
Smith, Duncan L
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Abstract
Identification of sites of protein SUMOylation is of great importance due its functional diversity within the cell. To date, most approaches to this problem rely on site-directed mutagenesis and/or highly specialised mass spectrometry approaches. We present a novel alternative approach to the site mapping of SUMOylation using trypsin and elastase digestion, routine mass spectrometry and an unbiased isotag database searching strategy.
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Date
2013-01-15
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Citation
A novel approach to the analysis of SUMOylation with the independent use of trypsin and elastase digestion followed by database searching utilising consecutive residue addition to lysine. 2013, 27 (1):127-34 Rapid Commun Mass Spectrom