DNA analysis by flow cytometry, response to endocrine treatment and prognosis in advanced carcinoma of the breast.
Baildam, Andrew D Zaloudik, J Howell, Anthony Barnes, Diana M Turnbull, Lesley Swindell, Ric Moore, Michael Sellwood, R A
Baildam, Andrew D
Zaloudik, J
Howell, Anthony
Barnes, Diana M
Turnbull, Lesley
Swindell, Ric
Moore, Michael
Sellwood, R A
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Abstract
The relationship between DNA content of mammary cancer and subsequent response to endocrine therapy was studied in 136 patients with advanced disease. All were treated with tamoxifen or ovarian ablation as first-line systemic therapy after relapse and were evaluable for response according to UICC criteria. DNA characterisation by flow cytometry was used on formalin fixed paraffin-embedded samples of tumour. Tumours were grouped according to DNA index into diploid (n = 52, 38%), 'tetraploid' (n = 46, 34%) and 'other DNA-aneuploid' (n = 38, 28%). The highest proportion of oestrogen receptor positive tumours (ER + ve) was found in the 'tetraploid' tumours (38/46, 85%, Chi-square = 8.53, P less than 0.02), and response rates, (SD + PR + CR), were 26/52 (50%), 34/46 (74%), and 15/38 (39%) respectively (Chi-square = 10.88, P less than 0.005). Patients with diploid or 'tetraploid' tumours survived longer and stayed in remission longer than those with 'other DNA-aneuploid' tumours. We suggest that 'tetraploid' or 'near tetraploid' human mammary tumours may comprise a distinct group of endocrine responsive tumours within the overall group of aneuploid tumours. The conventional interpretation of DNA histograms, grouping into diploid and aneuploid, may be masking important features of some tumour groups.
Affiliation
Description
Date
1987-05
Publisher
Keywords
Breast Cancer
Cancer DNA
Oestrogen Receptors
Cancer DNA
Oestrogen Receptors
Type
Article
Citation
DNA analysis by flow cytometry, response to endocrine treatment and prognosis in advanced carcinoma of the breast. 1987, 55 (5):553-9 Br. J. Cancer