A chromosome 14q11/TCR alpha/delta specific yeast artificial chromosome improves the detection rate and characterization of chromosome abnormalities in T-lymphoproliferative disorders.
Rack, K A Cornélis, F Radford-Weiss, I Bernheim, A Harrison, Christine J Hermine, O Prieur, M Vekemans, M Macintyre, E A
Rack, K A
Cornélis, F
Radford-Weiss, I
Bernheim, A
Harrison, Christine J
Hermine, O
Prieur, M
Vekemans, M
Macintyre, E A
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Abstract
The rate of detection of chromosome abnormalities in T-cell proliferations is lower than that observed in B-cell malignancies. The former frequently involve the TCR alpha/delta locus at chromosome band 14q11. We have identified a YAC encompassing 70% of the TCR alpha/delta locus, which has been used as a fluorescence in situ hybridization probe to detect chromosome rearrangements involving 14q11, both at metaphase and within interphase nuclei, in patients with a variety of T-lymphoproliferative disorders. Its use allowed detection of previously unsuspected TCR alpha/delta rearrangements in 4/13 (30%) immature T-lineage acute leukemias, including two t(10;14) and 2 minor inversion 14s. It also clarified interpretation of complex chromosome 14 abnormalities in mature T-cell proliferations (T-prolymphocytic leukemia and ataxia telangiectasia). Use of this probe will aid the detection and characterization of abnormalities involving the TCR alpha/delta locus, particularly in cases with normal or complex karyotypes and in those proliferations for which mitoses are difficult to obtain.
Description
Date
1997-08-01
Publisher
Collections
Keywords
T-Cell Leukaemia
T-Cell Lymphoma
Cancerous Stem Cells
T-Cell Lymphoma
Cancerous Stem Cells
Type
Article
Citation
A chromosome 14q11/TCR alpha/delta specific yeast artificial chromosome improves the detection rate and characterization of chromosome abnormalities in T-lymphoproliferative disorders. 1997, 90 (3):1233-40 Blood