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O6-alkylguanine-DNA alkyltransferase activity in schistosomiasis-associated human bladder cancer.
Badawi, A F Cooper, Donald P Mostafa, M H Aboul-Azm, T Barnard, R J Margison, Geoffrey P O'Connor, Peter J
Badawi, A F
Cooper, Donald P
Mostafa, M H
Aboul-Azm, T
Barnard, R J
Margison, Geoffrey P
O'Connor, Peter J
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Abstract
O6-Alkylguanine-DNA-alkyltransferase (ATase) activity was measured in extracts of 55 bladder tissue samples (46 tumour and nine uninvolved mucosal tissue) from Egyptian patients with schistosome-associated bladder carcinoma. Activity varied from 2.0 to 16.2 fmole ATase/microgram DNA (mean +/- S.D.; 5.6 +/- 4.0) or from 28 to 351 fmole ATase/mg (117 +/- 71). ATase levels in schistosome-associated bladder cancer tissues (5.6 +/- 4.0 fmole ATase/microgram DNA) tended to be lower than those observed in normal human bladder mucosal tissue (8.5 +/- 4.4 fmole ATase/microgram DNA). In a previous study (Badawi et al., Carcinogenesis, 1992, 13, 877-881) DNA-alkylation damage (O6-methyldeoxyguanosine) was found in 44/46 of these schistosome-associated bladder cancer samples at levels ranging from 0.012 to 0.485 mumole O6-MedG/mole deoxyguanosine. We now report an inverse correlation between the levels of methylation damage and ATase activity (r = -0.67; P < 0.001). These observations encourage further investigations of the possible role of environmental alkylating agents in the aetiology of early bladder cancer associated with schistosomiasis.
Description
Date
1994
Publisher
Collections
Keywords
Cancer DNA
Cancer Proteins
Urinary Bladder Cancer
Cancer Proteins
Urinary Bladder Cancer
Type
Article
Citation
O6-alkylguanine-DNA alkyltransferase activity in schistosomiasis-associated human bladder cancer. 1994, 30A (9):1314-9 Eur. J. Cancer