Identification of a putative intestinal stem cell and early lineage marker; musashi-1.
Potten, Christopher S Booth, Catherine Tudor, Gregory L Booth, Dawn Brady, Ged Hurley, Patricia Ashton, Garry Clarke, Robert B Sakakibara, Shin-ichi Okano, Hideyuki
Potten, Christopher S
Booth, Catherine
Tudor, Gregory L
Booth, Dawn
Brady, Ged
Hurley, Patricia
Ashton, Garry
Clarke, Robert B
Sakakibara, Shin-ichi
Okano, Hideyuki
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Abstract
There are few reliable markers for adult stem cells and none for those of the intestinal epithelium. Previously, indirect experimental approaches have predicted stem cell position and numbers. The Musashi-1 (Msi-1) gene encodes an RNA binding protein associated with asymmetric divisions in neural progenitor cells. Two-day-old, adult, and 4.5 h, 1-, 2-, 4- and 12-day post-irradiation samples of BDF1 mouse small intestine, together with some samples of mouse colon were stained with a rat monoclonal antibody to Musashi-1 (14 H-1). Min ( + / - ) mice with small intestinal adenomas of varying sizes were also analysed. Samples of human small and large bowel were also studied but the antibody staining was weak. Musashi-1 expression was observed using immunohistochemistry in neonatal, adult, and regenerating crypts with a staining pattern consistent with the predicted number and distribution of early lineage cells including the functional stem cells in these situations. Early dysplastic crypts and adenomas were also strongly Musashi-1 positive. In situ hybridization studies showed similar expression patterns for the Musashi mRNA and real-time quantitative RT-PCR showed dramatically more Msi-1 mRNA expression in Min tumours compared with adjacent normal tissue. These observations suggest that Musashi-1 is a marker of stem and early lineage progenitor cells in murine intestinal tissue.
Description
Date
2003-01
Publisher
Collections
Keywords
Intestinal Cancer
Type
Article
Citation
Identification of a putative intestinal stem cell and early lineage marker; musashi-1. 2003, 71 (1):28-41 Differentiation