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Technical report: SYBR Green I and the improved sensitivity of the single-cell electrophoresis assay.

Ward, Timothy H
; 
Marples, Brian
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Abstract
The single-cell electrophoresis (comet) assay is an established method for measuring radiation-induced strand breaks in DNA. The extent of damage is quantified in individual cells by assessing the intensity and distribution of a fluorescent DNA-binding dye using image analysis software. Using the Kinetic Imaging Komet3 system, it is demonstrated that the fluorochrome SYBR Green I improves the resolution and sensitivity of the comet assay, particularly for measuring radiation-induced double strand breaks.
Authors
Ward, Timothy H
Marples, Brian
Affiliation
Cancer Research Campaign Section of Drug Development and Imaging, Paterson Institute for Cancer Research, Christie Hospital (NHS) Trust, Manchester, UK.
Description
Date
2000-01
Publisher
Collections
All Paterson Institute for Cancer Research
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Keywords
Cultured Tumour Cells
Type
Article
Citation
Technical report: SYBR Green I and the improved sensitivity of the single-cell electrophoresis assay. 2000, 76 (1):61-5 Int. J. Radiat. Biol.
Journal Title
Journal ISSN
Volume Title
URI
http://hdl.handle.net/10541/90139
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