Intra-tumoral heterogeneity of tumour potential doubling times (Tpot) in colorectal cancer.

2.50
Hdl Handle:
http://hdl.handle.net/10541/99951
Title:
Intra-tumoral heterogeneity of tumour potential doubling times (Tpot) in colorectal cancer.
Authors:
Wilson, Malcolm S; West, Catharine M L; Wilson, G D; Roberts, Stephen A; James, Roger D; Schofield, Philip F
Abstract:
Intra-tumoural heterogeneity of proliferation has been assessed by taking multiple biopsies from 30 colorectal cancers. Following in vivo IUDR labelling, dual parameter flow cytometry was used to measure tumour DNA index (DI) and labelling index (LI) and to derive DNA synthesis time (Ts) and potential doubling time (Tpot). Heterogeneity was seen for all parameters under investigation. Overall coefficients of variation (CV) and logarithmic transformation of Ts and Tpot (due to their non-gaussian distributions) indicate that LI (CV 25%) was the most variable parameter. Intra-tumoral heterogeneity in Tpot (lnTpot CV = 22%) was less than inter-individual variation (CV = 63%), suggesting that this variation should not be a limitation to the possible usefulness of this technique as an independent prognostic indicator. Correlations of Tpot values were examined between the shortest, the median and the value for a pooled homogenate sample from a single tumour. Using an homogenate, it was possible to accurately predict classification of tumour Tpot values as being below the median ('fast tumours') in 15 of 19 cases (79%). The data suggest that assaying an homogenate may allow a more rapid analysis of a multiply sampled tumour.
Affiliation:
Clinical Research Department, Christie Hospital NHS Trust, Manchester, UK.
Citation:
Intra-tumoral heterogeneity of tumour potential doubling times (Tpot) in colorectal cancer. 1993, 68 (3):501-6 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Sep-1993
URI:
http://hdl.handle.net/10541/99951
PubMed ID:
8353040
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWilson, Malcolm Sen
dc.contributor.authorWest, Catharine M Len
dc.contributor.authorWilson, G Den
dc.contributor.authorRoberts, Stephen Aen
dc.contributor.authorJames, Roger Den
dc.contributor.authorSchofield, Philip Fen
dc.date.accessioned2010-05-27T15:41:00Z-
dc.date.available2010-05-27T15:41:00Z-
dc.date.issued1993-09-
dc.identifier.citationIntra-tumoral heterogeneity of tumour potential doubling times (Tpot) in colorectal cancer. 1993, 68 (3):501-6 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid8353040-
dc.identifier.urihttp://hdl.handle.net/10541/99951-
dc.description.abstractIntra-tumoural heterogeneity of proliferation has been assessed by taking multiple biopsies from 30 colorectal cancers. Following in vivo IUDR labelling, dual parameter flow cytometry was used to measure tumour DNA index (DI) and labelling index (LI) and to derive DNA synthesis time (Ts) and potential doubling time (Tpot). Heterogeneity was seen for all parameters under investigation. Overall coefficients of variation (CV) and logarithmic transformation of Ts and Tpot (due to their non-gaussian distributions) indicate that LI (CV 25%) was the most variable parameter. Intra-tumoral heterogeneity in Tpot (lnTpot CV = 22%) was less than inter-individual variation (CV = 63%), suggesting that this variation should not be a limitation to the possible usefulness of this technique as an independent prognostic indicator. Correlations of Tpot values were examined between the shortest, the median and the value for a pooled homogenate sample from a single tumour. Using an homogenate, it was possible to accurately predict classification of tumour Tpot values as being below the median ('fast tumours') in 15 of 19 cases (79%). The data suggest that assaying an homogenate may allow a more rapid analysis of a multiply sampled tumour.en
dc.language.isoenen
dc.subjectColorectal Canceren
dc.subjectCancer DNAen
dc.subject.meshAdenocarcinoma-
dc.subject.meshBiopsy-
dc.subject.meshCell Division-
dc.subject.meshColorectal Neoplasms-
dc.subject.meshDNA, Neoplasm-
dc.subject.meshFlow Cytometry-
dc.subject.meshHumans-
dc.subject.meshIdoxuridine-
dc.subject.meshPrognosis-
dc.titleIntra-tumoral heterogeneity of tumour potential doubling times (Tpot) in colorectal cancer.en
dc.typeArticleen
dc.contributor.departmentClinical Research Department, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren

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